Journal: Molecular medicine reports

Article Title: CXC195 induces apoptosis and endoplastic reticulum stress in human hepatocellular carcinoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.

doi: 10.3892/mmr.2015.4479

Figure Lengend Snippet: Figure 5. Effects of CXC195 on endoplasmic reticulum stress in the HepG2 cells. The HepG2 cells were treated with 150 µM CXC195 for 24 h. (A) Expression levels of GRP78, GRP94 and CHOP were measured in the HepG2 cells using western blot analysis. (B) Expression levels of p‑PERK, PERK, p‑eIF2α, eIF2α and ATF4 were measured in the HepG2 cells using western blot analysis. (C) Expression levels of IRE1α, p‑ASK, ASK, p‑p38, p38, p‑JNK and JNK were measured in the HepG2 cells using western blot analysis. (D) Expression of cleaved ATF6 was measured in the HepG2 cells using western blot analysis. GAPDH was used to confirm equal protein loading. Data are presented as the mean ± standard deviation (n=6). **P<0.01, vs. control. GRP, glucose‑regulated protein; CHOP, CCAAT‑enhancer‑binding protein homologous protein; p‑, phosphorylated; PERK, protein kinase R‑like endoplasmic reticulum kinase; eIF2α, eukaryotic translation initiation factor 2α; AIF4, apoptosis‑inducing factor 4; IRE1α, inositol‑requiring enzyme 1α; ASK, apoptosis signal‑regulating kinase; JNK, c‑Jun N‑terminal kinase; ATF6, activating transcription factor 6; Con, control.

Article Snippet: The membrane was blocked with 5% skimmed milk and incubated with PBS containing Tween 20 and the following primary antibodies at 1:500 dilution: Monoclonal/polyclonal mouse/rabbit anti-human proliferating cell nuclear antigen (PCNA) (cat no. sc-25280), p21 (sc-397), p27 (sc-393380), p53 (sc-126), Bcl-2 (sc-7382), Bcl-2-associated X (Bax) (sc-7480), AIF (sc-13116), Cyt-c (sc-13561), cyclooxygenase (COX)4 (sc-292052), tubulin (sc-9104), histone (sc-10806), glucose‐regulated protein (GRP)94 (sc-11402), GRP78 (sc-1050), CCAAT-enhancer-binding protein homologous protein (CHOP) (sc-575) and GAPDH (sc-365062) (Santa Cruz Biotechnology, Dallas, TX, USA) and cyclin-dependend kinase (CDK)4 (cat no. 12790), cyclin D1 (1044), phosphorylated (p)-PERK (3179), PERK (3192), eIF2α (9722), p-eIF2α (3597), ATF4 (11815), IRE1α (3294), p-ASK (3765), ASK (37626S), p-p38 (4511), p38 (9213), ATF6 (11815S), p-PI3K (4288S), PI3K (4249S), p-AKT (4060P), AKT (#2920S), mammalian target of rapamycin (mTOR) (2983S) and p-mTOR (5536S) (Cell Signaling Technology, Inc., Danvers, MA, USA) overnight at 4 ̊C.

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: Journal of thoracic disease

Article Title: 4-phenylbutyric acid re-trafficking hERG/G572R channel protein by modulating the endoplasmic reticulum stress-associated chaperones and endoplasmic reticulum-associated degradation gene.

doi: 10.21037/jtd-23-1252

Figure Lengend Snippet: Figure 4 4-PBA inhibited the expression of ATF6, GRP78, GRP94, and CRT/CNX. (A) WB results showing protein expression of ATF6,

Article Snippet: ATF6 rabbit polyclonal antibody (1:3,000; cat. no. 24169- 1-AP), GRP78/BIP rabbit polyclonal antibody (1:3,000; cat. no. 11587-1-AP), GRP94 rabbit polyclonal antibody (1:3,000; cat. no. 14700-1-AP), and HRD1/SYVN1 rabbit polyclonal antibody (1:3,000; cat. no. 13473-1-AP) were purchased from Proteintech® (Wuhan, China).

Techniques: Expressing